Isolation of microbial nucleic acids. Extraction of microbial RNA from aquatic sources
Extraction of microbial DNA from bulk soil
Extraction of DNA from phytoshpere, rhizosphere, rhizoplane
Extraction of RNA from bulk soil
Nucleic acid extraction from cultures
Detection of microbial nucleic acid sequences. Preparation of radioactive probes
Preparation of non-radioactive probes
Detection of microbial DNA sequences by colony hybridization
Detection and quantification of microbial nucleic acid seque Polymerase Chain Reaction
Identification and classification of microbes using DNA and RNA sequences. Partial and complete 16S rDNA sequences, their use in gneration of 16S rDNA phylogenetic trees and their implications in molecular ecological studies.
Microbial idenfication and design of phylogenetic trees based
Detection, identification and classification of microbes using other methods. Detection of gene transfer in the environment. Gene transfer by transformation
Gene transfer by conjugation
Gene transfer by transduction
Tracking of specific microbes in the environment. Marker genes
Designing field and microcosm experiments with GEM's.
